Figure 10. The effect of inhibitors on cell proliferation mediated by FGF-2 in CECs

The first passage CECs were plated into a 96-well plate and treated with one of the following conditions for 24 h when cells reached 80% confluency: FGF-2, FGF-2 and genistein in concentration ranging from 10 to 100 µM (top panel); FGF-2 and LY294002 in concentration ranging from 5 to 50 µM (middle panel); and wortmannin in concentration ranging from 10 to 500 nM (bottom panel). The cell proliferation assay was performed using CellTiter 96^RAQueous One Solution Cell Proliferation Assay (Promega) as described in the text. Inhibitory activity of the inhibitors were presented as percent of FGF-2 mediated cell proliferation.