Table 2 of
Rozsa, Mol Vis 4:20, 1998.Table 2 of
Rozsa, Mol Vis 4:20, 1998.
Table 2. Oligonucleotides used for PCR, Sequencing, and ASO in this work
Table 2A. Primers sets used to amplify TIGR exons for sequencing
Forward Reverse Family Use Fragment 5' GCTTTCCAGAGGAAGCCTCACC 5' CTCCAGAACTGACTTGTCTC All Exon 1 A 5' AATTGACCTTGGACCAGG 5' CACTGGCCCCTTCTCAGCCTTGCT All Exon 1 B 5' GCGACTAAGGCAAGAAAATG 5' AGGTCACTACGAGCCATATC All All Exon 1 C 5' ACATAGTCAATCCTTGGGCC 5' ATGAATAAAGACCATGTGGG All Exon 2 D 5' GGATTAAGTGGTGCTTCG 5' GCATAAACTGGCTGATGAG All Exon 3 E 5' AATTACTGGCAAGTATGGT 5' CAATGTCCGTGTAGCCAC All Exon 3 F 5' GGGTGCTGTGGTGTACTC 5' CATGCTGCTGTACTTATAGCG All Exon 3 G 5' GAACTCGAACAAACCTGGGAG 5' GAGCTATTCTGCTTCCT All Exon 3 H |
Table 2B. Primer sets used to amplify TIGR fragments for ASO and restriction analysis
Forward Reverse Family Use Fragment
5' AAGGTTCACATACTGCCTAG 5' GAAAGCAGTCAAAGCTGCC JG1 ASO I
JG3 ASO I
GL57(P) ASO I
5' CTGGCTCTGCCAAGCTTCCGCATGA 5' GGCTGGCTCTCCCTTCAGCCTGCT JG5 ASO J
5' AATTACTGGCAAGTATGGT 5' CATGCTGCTGTACTTATAGCG JG1 AlwNI K
GL7 AlwNI K
5' CACCCAGGAGACCACGTGGAGAATC 5' GAGGCCTGCTTCATCCACAGCCAAG GL57(M) BsrI L
----
(P): Tyr347Tyr polymorphism in UM:GL57; (M): Glu323Lys mutation in UM:GL57
|
Table 2C. Oligonucleotides used to sequence mutations
Oligonucleotide Family Use Fragment 5' GGGTGCTGTGGTGTACT JG1 Sequence I 5' TTGGCTGTGGATGAAGCA JG3 Sequence I 5' CTGGCTCTGCCAAGCTTCCGCATGA JG5 Sequence J 5' AATTACTGGCAAGTATGGT GL7 Sequence K 5' AAGGTTCACATACTGCCTAG GL57(P) Sequence I 5' TATGACAGTTCTGGACTCAGC GL57(M) Sequence L ---- (P): Tyr347Tyr polymorphism in UM:GL57; (M): Glu323Lys mutation in UM:GL57 |
Table 2D. Oligonucleotides used for ASO hybridization
Oligonucleotide Name Family Use Fragment 5' GACAGTTCCCGTATTCTTG 370PRO JG1 Control I 5' GACAGTTCCTGTATTCTTG 370LEU JG1 Mutant I 5' AAGCAGTCAGTCGCCAATG 426VAL JG3 Control I 5' AAGCAGTCATTCGCCAATG 426PHE JG3 Mutant I 5' GTTTGGGTAGGAGAGCCT 252GLY JG5 Control J 5' GTTTGGGTAAGAGAGCCT 252ARG JG5 Mutant J 5' CATAAGATATGAGCTGAAT c347TYR GL57(P) Control I 5' CATAAGATACGAGCTGAAT m347TYR GL57(P) Mutant I ---- (P): Tyr347Tyr polymorphism in UM:GL57 |