Figure 5. TIGR/MYOC polymorphisms in two glaucoma probands shown by dye-terminator sequencing

Chromatograms of a segment of the TIGR/MYOC gene containing the Gly252Arg and Glu323Lys mutations for UM:JG5 and UM:GL57, respectively. Samples were sequenced using an ABI377 semi-automated sequencer using dye-terminator chemistry. Locations of the mutated bases are shown with an arrowhead and the corresponding change in amino acid sequence is shown above.