Figure 8. Electrophoretic Mobility Shift Assay (EMSA) of AEE-like or TonE-like elements with whole cell extracts from [alpha]TN4 cells

Free AEE-like probe is shown in lane 1. Complexes, indicated by arrows, are seen for AEE-like (A, B, C) and to a lesser extent for TonE-like (A, B) radiolabelled oligomers under non-stressed (NonStr) and osmotically-stressed (Str) conditions (lanes 2, 3, 6, and 7). Radiolabelled oligomers lacking only the AEE-like or TonE-like sites failed to substantially bind trans-acting factors under nonstressed and osmotically-stressed conditions (lanes 4, 5, 8, and 9). Free unbound probe is shown by arrows (D,E).