Figure 3. Primer extension of mouse AR transcription start site

Total RNA from different tissues was hybridized with a kinase-labelled mouse AR exon 1 specific primer and reverse transcribed, terminating at the cap site. The GATC sequence ladder to ascertain nucleotide length is shown along with results from brain, testis, and yeast (control). The major product, which is approximately 74 bp in size and terminates at a cytosine residue, is specified by an arrow and shown in the autoradiograph.