Figure 3. Re-confirmation of osteonectin over-expression between pooled cataract and increasing amounts of normal RNA

Ethidium bromide stained gel showing the levels of osteonectin and [alpha]B-crystallin after 35 PCR cycles between 50 ng (1X) of pooled cataract RNA, C, (lanes 5 and 10) and increasing amounts 50 ng (1X), lanes 4 and 9; 100 ng (2X), (lanes 3 and 8); 250 ng (5X), (lanes 2 and 7); and 500 ng (10X), (lanes 1 and 6) of normal (N) RNA. Underlined are the 419 bp osteonectin product (lanes 1-5) and the 547 bp [alpha]B-crystallin control product (lanes 6-10).