Figure 1 of
Zhu, Mol Vis 1998;
Figure 1. Summary of the yeast two-hybrid library screen
A 100 micrograms of a bovine retinal cDNA library plasmids in yeast expression vector pGAD10 was transformed into yeast strain CG-1945 co-expressing BD-PhLOP1 hybrid protein. One out of 2.5 X 104 yeast transformants screened exhibited transactivation of both HIS3 and LacZ reporter genes. Of the 7 positive clones, 5 were characterized and identified as bSUG1.