Figure 1 of Zhu, Mol Vis 1998; 4:13.

Figure 1. Summary of the yeast two-hybrid library screen

A 100 micrograms of a bovine retinal cDNA library plasmids in yeast expression vector pGAD10 was transformed into yeast strain CG-1945 co-expressing BD-PhLOP1 hybrid protein. One out of 2.5 X 104 yeast transformants screened exhibited transactivation of both HIS3 and LacZ reporter genes. Of the 7 positive clones, 5 were characterized and identified as bSUG1.

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