Figure 3 of
Chitpinityol, Mol Vis 4:1, 1998.
Figure 3. Comparison of milk clotting activity of chymosin and mutant using microtitre plate assay.
Twenty-five µl of activated chymosin and mutants were 1:1 serial diluted with 12% skim-milk solution and incubated at 37 °C, 25 min. Activity calculates from the number of clotted-milk-wells after inverting the plate. Row " + ": 4 µg of authentic chymosin in the first well, Row " - ": negative control, Row "C": wild-type chymosin, Row "C+2": the mutant PC+2-chymosin. See text for details.