Figure 2. Stability of [alpha]-crystallin under refolding conditions.

Panel (a): Non-denaturing PAGE gel; Panel (b): Western blot probed with polyclonal anti-[alpha]-crystallin antibodies. Lane 1, [alpha]-crystallin after incubation in phosphate buffer, pH 10.7; Lane 2, [alpha]-crystallin after incubation in phosphate buffer, pH 8.0; Lane 3, [alpha]-crystallin after dialysis; Lane 4, [alpha]-crystallin after incubation at pH 2.0; Lane 5, [alpha]-crystallin after incubation at pH 6.3 and centrifugation.