Molecular Vision: Ashander, Mol Vis 2026; 32:34-47. Table 1

Table 1. Sequences and amplicon sizes of primer pairs used in quantitative polymerase chain reactions.

Gene transcript [Reference]	Primer sequences (5’-3’)	Size (bp)	NCBI accession number
Immune Response
CCL2^✝	F: GCATGAAAGTCTCTGCCGC R: GGACACTTGCTGCTGGTGATT	181	NM_002982.4
CD274 [49]	F: ACGCATTTACTGTCACGGTTCC R: GACTTCGGCCTTGGGGTAGC	446	NM_014143.4
EIF2AK2 [50]	F: GGAAAGCGAACAAGGAGTAAGG R: CCAAAGCGTAGAGGTCCACT	97	NM_002759.4
ICAM1 [51]	F: TAAGCCAAGAGGAAGGAGCA R: CATATCATCAAGGGTTGGGG	289	NM_000201.3
IFIT1 [52]	F: AGAAGCAGGCAATCACAGAAAA R: CTGAAACCGACCATAGTGGAAAT	112	NM_001548.5
IFITM1 [53]	F: ACTCCGTGAAGTCTAGGGACA R: TGTCACAGAGCCGAATACCAG	155	NM_003641.5
IL1B [49]	F: TGACCTGAGCACCTTCTTTC R: CAGCTGTAGAGTGGGCTTATC	331	NM_000576.3
IL1RN [54]	F: TCATGCTCTGTTCTTGGGAAT R: GCTTGTCCTGCTTTCTGTTC	131	NM_173842.3
IL6 [55]	F: ATGCAATAACCACCCCTGACC R: CCATGCTACATTTGCCGAAGAG	160	NM_00600.5
ISG15 [56]	F: GAGAGGCAGCGAACTCATCT R: AGCATCTTCACCGTCAGGTC	99	NM_005101.4
MX1 [57]	F: ACAATCAGCCTGGTGGTGGTC R: CCTCCCCTACAGTTTCCTCTCC	565	NM_001144925.2
OAS1 [57]	F: AGGTGGTAAAGGGTGGCT R: TGCTTGACTAGGCGGATG	472	NM_016816.4
PDCD1LG2 [58]	F: CAACTTGGCTGCTTCACATTTT R: TGTGGTGACAGGTCTTTTTGTTGT	137	NM_025239.4
RIGI [59]	F: GACTGGACGTGGCAAAACAA R: TTGAATGCATCCAATATACACTTCTG	75	NM_014314.4
RSAD2 [60]	F: TGACGGAACAGATCAAAGCA R: GCACCAAGCAGGACACTTCT	174	NM_080657.5
TGFB1^✝	F: CGTGGAGCTGTACCAGAAATAC R: CGTGGAGCTGAAGCAATAGT	367	NM_000660.7
TGFB2^✝	F: GTACTACGCCAAGGAGGTTTAC R: TGTGGAGGTGCCATCAATAC	568	NM_001135599.2
TNF [61]	F: TCTCGAACCCCGAGTGACAA R: TGAAGAGGACCTGGGAGTAG	181	NM_000594.4
VCAM1^✝	F: GGATGCAGACAGGAAGTCCC R: GCTGGAACAGGTCATGGTCA	247	NM_001078.4
Reference
GAPDH [62]	F: AGCTGAACGGGAAGCTCACTGG R: GGAGTGGGTGTCGCTGTTGAAGTC	209	NM_002046.7
PPIA [28]	F: GAGCACTGGAGAGAAAGGATTT R: GGTGATCTTCTTGCTGGTCTT	355	NM_021130.5

Abbreviations: CCL2=C-C motif chemokine ligand 2, EIF2AK2=eukaryotic translation initiation factor 2 alpha kinase 2, GAPDH=glyceraldehyde-3-phosphate dehydrogenase, ICAM1=intercellular adhesion molecule 1, IFIT1=interferon induced protein with tetratricopeptide repeats 1, IFITM=interferon induced transmembrane protein 1, IL1B=interleukin 1 beta, IL1RN=interleukin 1 receptor antagonist, IL6=interleukin 6, ISG15=ISG15 ubiquitin like modifier, MX1=MX dynamin like GTPase 1, OAS1=2'-5′-oligoadenylate synthetase 1, CD274=CD274 molecule (programmed death-ligand 1), PDCD1LG2=programmed cell death 1 ligand 2, PPIA=peptidylprolyl isomerase A, RIGI=RNA sensor RIG-I, RSAD2=radical S-adenosyl methionine domain containing 2, TGFB1=transforming growth factor beta 1, TGFB2=transforming growth factor beta 2, TNF=tumor necrosis factor, VCAM1=vascular cell adhesion molecule 1. ^✝Primers designed in-house and amplicons confirmed by sequencing.