Figure 2. CRISPR-generated Bicdl1 KI mice reconstitute the phenotype discovered in the mutagenesis screen. A Bicdl1 KI mouse carrying a replacement allele for the bargain mutation was generated by CRISPR mutagenesis. A-F: Representative OCT images at 3 weeks, 9 months, and 15 months of age show decreased ORT. G-I: Quantification of ORT (G), ONL (H) and TRT (I) thicknesses at different ages (G, H, I; WT n = 7, KI n = 5 at 3-week-old, WT n = 16, KI n = 20 at 2-month-old, WT n = 16, KI n = 17 at 4-month-old, WT n = 14, KI n = 17 at 6-month-old, WT n = 19, KI n = 19 at 9-month-old, WT n = 9, KI n = 10 at 12-month-old, WT n = 8, KI n = 8 at 15-month-old) in Bicdl1 WT and KI mice. A significant difference in ORT was observed between Bicdl1 WT and KI mice at all time points. ONL thinning starts at 6 months of age. Black squares: WT mice, blue squares: Bicdl1 KI mice, green-filled squares: male, open squares: female; green line: TRT, yellow line: ONL, blue line: ORT, ns: non-significant, #: p < 0.1, *: p < 0.05, **: p < 0.01, ***: p < 0.001, ****: p < 0.0001, scale bar: 100 µm.