Figure 4. Effect of SS31 on AMPK and Drp1 expression in 661W cells. A–C: Western blot (A) and density analysis (B, C) of 661W cell lysates treated with 24-h hydrogen peroxide (150 μM), SS31 (100 nM, 2 h) with or without pretreatment, AICAR (17.5 μM, 2 h), or dorsomorphin (7 μM, 2 h). Application of H₂O₂ significantly decreased the expression of AMPK and Drp1, while the addition of SS31 treatment on the basis of injury significantly increased the expression of AMPK and Drp1. *Significantly different from H₂O₂, p < 0.05. n = 6.