Figure 1. Pilot differentiation timeline and morphological progression of PGP1-hIPSCs toward photoreceptor lineage. A: Schematic of the pilot differentiation protocol. PGP1-hIPSCs were cultured in primary medium from Day 0 (D0) to D8 to induce eyefield progenitor cells (EFPCs), followed by neural induction medium from D9 to promote neural retina progenitor cells (NRPCs), with additional supplementation to guide photoreceptor (PhR) lineage specification. B: Overview of small molecules used during differentiation. Supplementation included T3 (D9), DAPT (D19), taurine (D23), and retinoic acid (D33). Full medium composition is provided in Appendix 1 and Appendix 2. C: Representative cell morphology from D0 (i.e., IPSCs) to D3 in primary medium compared to D20 in secondary medium, where neurite outgrowth is evident. D: Live fluorescence imaging at D27 revealed RCVRN-mCherry reporter expression, indicating photoreceptor lineage commitment.