Figure 8. Effects of TLR9 inhibiting oligonucleotides on the viability of cultured human RPE cells under hyperosmotic conditions and hypoxic conditions (CoCl₂, 0.1% O₂). The cells were cultured 24 h in absence and presence of high NaCl (+100 mM), CoCl₂ (150 µM), as well as 0.1% O₂ with and without inhibitory oligonucleotides specific to TLR9. A: Effect of the inhibitory oligonucleotide iODN 2088 (1 µM) on the viability of cultured RPE cells. n=7 (NaCl), n=6 (CoCl₂), and n=4 (0.1% O₂). B: Effect of the TLR9 inhibitory oligonucleotide iODN INH-18 (2.5 µM) on the viability of cultured RPE cells (n=6 for NaCl and CoCl₂), and n=4 (0.1% O₂). C: Influence of chemical hypoxia induced by CoCl_2, of a 0.1% O₂ atmosphere or of high extracellular osmolarity on necrosis and apoptosis in cultured RPE cells (n=4). The data are shown as percent of unstimulated control (100%). Each bar represents data obtained in independent experiments using cells from different donors. Mann–Whitney-U-Test was used for statistical analysis. Significant difference versus unstimulated control: *p<0.05. Significant differences versus 0.1% O₂ controls: ●p<0.05. .