Figure 4. mtDNA levels increase in response to stress in healthy cybrids but not in DAMD cybrids. Healthy and DAMD cybrids were treated with 1 uM FCCP for 4 h daily for 0, 1, 2, and 3 days to simulate metabolic insult and bioenergetic stress conditions. Healthy cybrid mtDNA levels significantly correlated with metabolic stress (p=0.0012), while DAMD cybrid mtDNA showed no correlation (p=0.75). However, when compared, there was no significant difference in the absolute increase in mtDNA between healthy and DAMD cybrids (C). Healthy Cybrids: n=5, DAMD Cybrids: n=5. .