Figure 3. Mitochondrial mass and electron transport chain proteins were unchanged between healthy and DAMD cybrids. western blotting of TOM20 (A), a ubiquitously expressed transport protein subunit, was used to quantify total mitochondrial mass, which was unchanged between healthy and DAMD cybrids (B). To assess mitochondrial protein expression, western blotting of several ETC proteins was performed (C). There was no difference found between healthy and DAMD cybrids in protein expression of SDHB of complex II (D), UQCRC2 of complex III (E), COX II of complex IV (F), and ATP5 of complex V (G). Healthy Cybrids: n=5, DAMD Cybrids: n=5. .