Figure 5. Expression and safety of shEgr1 and mEgr1 transgenes in wt cones. (A-C) Fundus images and OCT scans (top rows) and immunofluorescence images (bottom rows) of wt retinas subretinally injected with (A) AAV::OPN1MW-ctrl, (B) AAV::OPN1MW-shEgr1 and (C) AAV::OPN1MW-mEgr1 at 8 wpi. White arrows point to injection sites. OCT scans are color-coded in red when across the optic nerve or blue when across the injection site. Immunofluorescence panels show the transduction pattern of the virus (GFP, except AAV::OPN1MW-mEgr1) as well as EGR1 and PNA-positive cells as grayscale images and the colored merge. Scale bars: 20 μm. D) Left panel: example image of a cone arrestin (ARR3) staining in wt mice. Right panel: bar graph reporting the number of ARR3-positive cells in a 600 μm-long region of the transduced and untransduced retinal areas of wt mice at 8 wpi of AAV::mOP-ctrl (red), AAV::mOP-shEgr1 (blue), and AAV::mOP-mEgr1 (green). Shown are individual values and the mean ± SD. Two-way ANOVA with Šídák's multiple comparisons test. n=3. ONL: outer nuclear layer; INL: inner nuclear layer; GCL: ganglion cell layer.