Figure 1. Defective DNA methyltransferase 1 expression and DNA methylation in the ocular surface epithelium of Dnmt1^ΔOSE embryos. Wild-type and Dnmt1^ΔOSE E15.5 embryonic eye sections were examined by immunostaining for (A) DNMT1 and (B) 5meC. Dashed lines indicate the epithelial basement membrane. Top panels, low magnification images; middle and lower panels, higher magnifications of selected areas of (a) the eyelid and (b) the cornea, respectively. Arrowheads point to the eyelid epithelium in (a) and the corneal epithelium in (b), the asterisk marks the lens epithelium in (b). EL, eyelid; CO, cornea; RE, retina; LN, lens. Images are representative of at least three mice/genotypes. Scale bars: 50 µm.