Figure 5. Clustal Omega multiple-sequence alignment showing the evolutionary conservation of amino acids across species for which novel substitutions (highlighted red) were identified in this study. A: Conservation of serine at position 157 in the MYO7A protein. B: Conservation of glutamine at position 381 in the USH2A protein. C: Conservation of proline at position 116 in the ARL6 protein. D: Conservation of threonine at position 194 in the CLN5 protein.