To access the data, click or select the words “Appendix 2.” To induce IL-15 knockout, we employed the CRISPR/Cas9 technology. Initially, we designed and synthesized a single guide RNA (sgRNA) sequence targeting a conserved region within the coding region of the IL-15 gene. A–C. The knockout of the IL-15 gene in THP-1 was validated by PCR and western blot tests. This study designed three small interfering RNAs (siRNAs) for each STAT5, EFNA1, and NCOA2 mRNA. D–F. PCR was conducted to evaluate the efficiency of these siRNAs on the knockdown of STAT5, EFNA1, and NCOA2. *p < 0.05, **p < 0.01, ***p < 0.001 versus Con. #p < 0.05, ##p < 0.01, ###p < 0.001 versus IL-15. n = 3. Statistical significance was determined by using an unpaired Student t test for two groups or one-way analysis of variance for more than two groups. Subsequently, Dunnett’s post hoc tests were conducted. p < 0.05 was considered significant.