Figure 1. Experimental results of lentiviral infection of human retinal pigment epithelial cells (ARPE-19) at different multiplicities of infection (MOI = 0, 2.5, 5, 10, 20, 40; n=3). A: Observed under a 40× fluorescence microscope at 24 h and 48 h post-infection with lentiviruses of different MOIs, the MOI was positively correlated with fluorescence intensity. B: At 24h after lentiviral infection of ARPE-19 cells at different MOIs, the mRNA expression level of paired box gene 6 (PAX6) was inversely correlated with MOI, and no statistically significant difference in mRNA expression was observed when MOI>20. C, D: At 48h after lentiviral infection of ARPE-19 cells at different MOIs, the PAX6 protein expression level showed a decreasing trend, and no statistically significant difference in protein expression was detected when MOI > 20 (the MOI = 0 group exhibited statistically significant differences compared with all other groups; no statistically significant difference was observed between the MOI = 20 group and the MOI = 40 group; *p<0.05, **p<0.01, ***p<0.001).