Table 1 of
Kalahasti, Mol Vis 2025; 31:411-422. Table 1 of
Kalahasti, Mol Vis 2025; 31:411-422.
Table 1. Details of primary and secondary antibodies and their concentrations used for immunoblot and immunofluorescence experiments.
| Primary antibody | ||||
|---|---|---|---|---|
| Name | Cat. No | Concentration | Dilution | Application |
| GFAP | PA3–16727 | 2 mg/ml | 1:4000 | Immunofluorescence |
| Rhodopsin | MA1–722 | 1 mg/ml | 1:1000 | Immunofluorescence |
| AKR1B1 | PA5–29718 | 1.06 mg/ml | 1:1000 | Immunoblotting |
| Bax | 2772S | 112 µg/ml | 1:1000 | Immunoblotting |
| Bcl2 | MA5–11757 | 0.2 mg/ml | 1:500 | Immunoblotting |
| Caspase12 | PA5–19963 | 1 mg/ ml | 1:1000 | Immunoblotting |
| Caspase3 | 9662S | 26 µg/ml | 1:1000 | Immunoblotting |
| GAPDH | 2118S | 42 µg/ml | 1:1000 | Immunoblotting |
| HIF1α | ab187524 | 1.78 mg/ ml | 1:1000 | Immunoblotting |
| MCP1 | PA1–84244 | 1 mg/ml | 1:1000 | Immunoblotting |
| NF-κB | 8242S | 139 µg/ml | 1:1000 | Immunoblotting |
| pNF-kB | 3033S | 57 µg/ml | 1:1000 | Immunoblotting |
| TNFα | PA5–19810 | 0.9 mg/ml | 1:1000 | Immunoblotting |
| VEGF | MA1–16629 | 1 mg/ml | 1:1000 | Immunoblotting |
| β-Actin | MA1–91399 | 2.9 mg/ml | 1:5000 | Immunoblotting |
| Secondary antibody | ||||
| Anti-mouse | A9044 | 10.5 mg/ml | 1:10000 | Immunoblotting |
| Anti-Rabbit | A9169 | 16.1mg/ml | 1:10000 | Immunoblotting |
| Anti-mouse Alexa Flour 555 | A31570 | 2 mg/ml | 1:1000 | Immunofluorescence |
| Anti-Rabbit Alexa Flour 568 | A10042 | 2 mg/ml | 1:2000 | Immunofluorescence |