Figure 5. DN-NFAT5 does not affect MCP-1 concentration in the cell culture medium of ARPE-19 cells incubated with Na100. ARPE-19 cells transfected with an EGFP plasmid (control) or with DN-NFAT5 plasmid were incubated for 8 h with Iso or Na100. The cell supernatants were then collected and applied to CHO-WTA11 cells expressing CCR2 to measure calcium mobilization. The results were normalized for baseline activity (0%) and the maximal response obtained with 25 µM adenosine triphosphate (100%). MCP-1 concentration was then determined using a standard curve. MCP-1 concentration (nM) is the mean ± SEM of four independent experiments and measurements performed in duplicates. Data were analyzed using the one-way analysis of variance and post hoc Sidak’s tests. *p < 0.05, **p < 0.01.