Figure 3. sh-NFAT5 and DN-NFAT5 decrease the transactivation activity of NFAT5 in ARPE-19 cells. ARPE-19 cells were transfected with pSEAP-TonE plasmid and sh-CTN (control) or sh-NFAT5 (A) or with pSEAP-TonE plasmid and pEGFP (control) or DN-NFAT5 (B). Then, ARPE-19 cells were subjected to Iso (white columns) or Na100 (black columns) for 8 h, and SEAP activity was determined as described under Methods. Data are expressed as SEAP activity (in fold over control Na100 condition set to 1) and are the mean ± SEM of three independent experiments. Data were analyzed using the conformity t test and the paired t test. *p < 0.05, **p < 0.01, ***p < 0.001.