To access the data, click or select the words “Appendix 2.” Hyperosmolar stress-induced increase in NFAT5 mRNA is reduced by sh-NFAT5. ARPE-19 cells were transfected with Sh-CTN plasmid (Control) or Sh-NFAT5 plasmid; then subjected to Iso (white columns) or Na100 (black columns) for 8h as described previously. NFAT5 mRNA levels are expressed in fold expression over Iso set to 1 following normalization with appropriate reference genes. Data are the mean ± S.E.M. of 6 independent experiments. Data were analyzed using the conformity t-test and the paired t-test. *: p<0.05, **: p<0.01 and ***: p<0.001.