Figure 7. This figure shows confirmation of tubb4b morpholino knockdown. (A) Schematic representation of MO-1 splice-blocking that removes exon 2 (Ex 2) in the mutant (mt) mRNA transcript. FP refers to the forward primer; RP refers to the reverse primer; utr refers to the untranslated region. (B) Representative RT-PCR gel confirming reduction in size of tubb4b amplicon. MO1 refers to splice-blocking morpholino; SM refers to size marker; WT refers to the wild-type. (C) Representative image of a western blot of tubb4b protein in zebrafish injected with MO2 translation-blocking morpholino. The gel loading control is gapdh. MW refers to molecular weight size marker and WT refers to the wild-type. (D) Representative RT-PCR gels showing levels of rhodopsin (rho) and tubb6 transcripts with MO2 translation-blocking morpholino knockdown relative to gapdh expression.