Figure 5. AAV5-Pde6b treatment protected retinal cell layers and improved visual function in Pde6b knockout rats. A: Experimental scheme. B–D: Representative images of hematoxylin and eosin staining in the retina of wild-type (WT) rats (B) and Pde6b knockout (KO) rats injected with AAV5-GFP (C) or AAV5-Pde6b (D; n=4 for each group). E. Quantification of the retinal thicknesses. F–H: Representative images of in vivo optical coherence tomography scans of WT rats (F) and Pde6b KO rats injected with AAV5-GFP (G) or AAV5-Pde6b (H; n=4 for each group). I: Quantification of the thickness of the separated layer. J: Representative electroretinogram responses to light stimulus. K. Quantitative analysis of the scotopic A-wave and B-wave (n=4 for each group). L: Quantitative eye movement analysis by optokinetic nystagmus test (n=4 for each group). GCL, ganglion cell layer; IPL, inner plexiform; INL, inner nuclear layer; OPL, outer plexiform layer; ONL, outer nuclear layer; IS, inner segments; OS, outer segments; RPE, retinal pigment epithelium. Scale bar, 50 μM. Error bars, mean ± SEM, *p<0.01, ANOVA with Student–Newman–Keuls post hoc analysis.