Table 2 of
Lin, Mol Vis 2025; 31:1-9. Table 2 of
Lin, Mol Vis 2025; 31:1-9.
Table 2. Pitfalls and trouble shooting.
| Problems | Probable cause | Suggestions |
|---|---|---|
| Localized cornea haze | Repeated cornea injection | When multiple injections are needed, try to use the same incision site each time. |
| Bleeding | Traumatizing iris blood vessel | Make sure the pupil is fully dilated. Sweep close to the lens to avoid injuring the iris. |
| Cloudy lens | Nicking lens capsule | Avoid touching the lens with needle tips. |
| Retina damage | Penetrating the retina | When the needle is in the vitreous, avoid pushing the needle too far toward the retina. |
| Air bubbles | Syringe not degassed | Flush thoroughly with ethanol and PBS before filling with vector solution. Degas the solution in the syringe. |