Molecular Vision: Jiang, Mol Vis 2024; 30:466-476. Table 1

Table 1. qPCR analysis for PITX2 and neighboring genes.

ID	ENPEP-e12		PITX2-e4		FAM241A-e2		NDST4-e4		NDST4-e2
	Forward primer chr4:110563420-110563440 TCTTTTCACTCCACACGCGAA		Forward primer chr4:110632981-110633000 GCGGCAGTTGGTCTCCATTC		Forward primer chr4:112186789-112186810 AACATGGGCTTCACAAGGATGT		Forward primer chr4:114970370-114970392 CACTTCCACCACAAGATCACAAC		Forward primer chr4:115076717-115076737 TGGTACTGAAATCGGCTGGAC
	Reverse primer chr4:110563506-110563484 ACAGGTGTTGTTTGAGAAGGACC		Reverse primer chr4:110633082-110633061 ACGTAGTCTCATCTGAGCCCTG		Reverse primer chr4:112186879-112186859 GGCCAAGGAACCACAGCATAA		Reverse primer chr4:114970462-114970439 GAGCAGATGATTCTCAACAAGGAA		Reverse primer chr4:115076823-115076803 GACACATCCAAAACGGACCCT
	ACN		ACN		ACN		ACN		ACN
	ENPEP-e12/ POLR2A	ENPEP-e12/ RPP14	PITX2-e4/ POLR2A	PITX2-e4/ RPP14	FAM241A-e2/POLR2A	FAM241A-e2/RPP14	NDST4-e4/ POLR2A	NDST4-e4/ RPP14	NDST4-e2/ POLR2A	NDST4-e2/ RPP14
I-1	1.769	1.677	1.733	1.646	2.077	1.968	1.520	1.438	1.555	1.475
I-2	1.752	2.000	1.422	1.626	2.070	2.363	1.616	1.841	1.701	1.942
II-1	1.604	1.599	0.722	0.721	0.987	0.984	0.791	0.787	0.782	0.780
II-2	1.849	1.887	0.596	0.609	1.056	1.078	0.869	0.885	0.871	0.889
PC-1	2.041	2.162	1.906	2.024	2.034	2.155	2.167	2.291	1.987	2.106
PC-2	2.002	1.929	2.096	2.024	1.995	1.923	1.968	1.893	2.015	1.943
PC-3	1.958	1.909	1.997	1.952	1.971	1.923	1.865	1.815	1.998	1.951

ΔCt=Ct(Purpose segment)- Ct(Internal reference gene); Relative copy number=2^(-ΔCt); Absolute copy number (ACN)=(Test sample relative copy number / Relative copy number of control samples) *2; POLR2A and RPP14 genes are internal reference genes on autosomes, and we assumed their copy number to be 2 in all samples. PC-1, 2, and 3 are normal negative controls. Deleted sequences are in bold numbers. Primer design range according to NCBI database GRCh38.