Figure 3. Identification of conserved motif and validation of differential promoter binding via ChIP-qPCR. A: BMAL1 binding site motif enriched in our analysis, (B) ClueGo enrichment network depicts the functional roles associated with 115 genes bound to the BMAL1 binding site motif. C: Genomic tracks from USCS genomic browser showing binding activity at the upstream promoter regions of SHMT2, MALAT1, CIART, Per1, G6PC3, and TXNIP, (D) Bar chart showing validation of these targets using qRT-PCR, the number next to each gene represents bp upstream (-) or downstream (+) of the transcription start site; the Untrt12 (untreated 12) corresponds to a proprietary primer pair expected not to show any binding. MALAT1- metastasis-associated lung adenocarcinoma transcript 1, SHMT2- Serine Hydroxymethyltransferase 2, PER1-Period 1, TXNIP- thioredoxin interacting protein, G6PC3- glucose-6-phosphatase catalytic subunit 3, CIART-circadian-associated repressor of transcription.