Figure 4 of Vendra, Mol Vis 2024; 30:37-48.

Figure 4. Amyloid fibril detection of wild-type (black +) and Y134X mutant human γD-crystallin (brown circles) using Thioflavin-T. A: Thioflavin-T spectra at pH 7.3. Each spectrum is an average of three independent scans of three separate samples. B: Thioflavin-T fluorescence at pH 7.3 as a function of time. Values represent the mean of three independent experiments of three separate samples; error bars indicate the standard deviation. Protein concentration: 9 μM; λex: 444 nm; excitation and emission slits: 10 nm. If: Intensity of fluorescence.