Figure 3. Guanidine hydrochloride unfolding of wild-type (black squares) and Y134X mutant human γD-crystallin (brown circles). Protein samples were excited at 295 nm, and the relative emission intensity of the 350 nm fluorescence (denatured form) was compared to that of the 330 nm (native protein) and recorded as a function of the denaturant concentration. The line indicates the fitted data, and the blocks stand for raw data. Protein concentration: 0.1 mg/ml; excitation emission slits: 10 nm. The data were acquired from two independent experiments of two separate sets of samples.