Figure 2 of Vendra, Mol Vis 2024; 30:37-48.


Figure 2. Surface hydrophobicity measurement of wild-type (black squares) and Y134X mutant human γD-crystallin (brown circles). A: bis-ANS spectra. Values represent the mean of three independent experiments; error bars indicate the standard deviation. Protein concentration: 6 μM; λex: 390 nm; λem: 510 nm; excitation emission slits: 2.5 nm. B: Nile red spectra. The data are the average of three independent experiments of three separate samples. Error bars indicate the standard deviation. Protein concentration: 6 μM; λex: 540 nm; λem: 605 nm; excitation emission slits: 10 nm. If: fluorescence intensity.