Figure 2. WTAP siRNA downregulates WTAP expression and m6A modification levels. A: After injecting three WTAP siRNAs into the vitreous body, qRT-PCR analysis was used to detect the expression of WTAP. QRT-PCR analysis (B) and protein immunoblotting (C and D) were used to measure WTAP expression after intraocular injection of WTAP siRNA. E: Quantitative analysis suggests that compared to the 7-day group, the m6A level was reduced after WTAP siRNA was injected into the vitreous body.