Figure 2. Immunofluorescence of conjunctival epithelial cells showing higher levels of IL-5 and IL-6 in patients with keratoconus compared to the control group. A. Immunofluorescence of control conjunctival epithelial cells labeled for 4′,6-diamidino-2-phenylindole (DAPI, blue), IL-6 (green), IL-5 (red), and merge. B. Immunofluorescence of keratoconus conjunctival epithelial cells labeled for DAPI (blue), IL-6 (green), IL-5 (red), and merge (bars=20 um). C. Intracellular fluorescence intensity of IL-6. D. Intracellular fluorescence intensity of IL 5. ****p<0.0001.