Figure 1. Timeline of treatments and assessments. After baseline measurements, a high-fat diet (HFD) was given to the rats that would comprise the HFD and the HFD+streptozotocin (STZ) groups. After 4 weeks on HFD, the HFD+STZ group received 2–3 injections of STZ over the course of 2–3 weeks. The rats were then followed for 8 weeks with assessments performed as depicted above. The rats were euthanized at 10 weeks post-hyperglycemia. The assessments were optomotor response (OMR), Y-maze performance, glucose tolerance test (GTT), insulin tolerance test (ITT), and electroretinograms (ERG).