Figure 4. SFN suppresses the formation of stress fibers induced by TGF-β in HTFs. (A) After embedding with collagen gels, cells were incubated for 24 h with or without SFN, followed by a 24 h culture with or without TGF-β. The formation of stress fibers was determined using Alexa Fluor 568–labeled phalloidin and DAPI staining. Scale bars: 50 µm. Fluorescence intensity analysis in (A) is shown in (B). Data are presented as mean ± SD (n=3). *p<0.05 compared to controls. †p<0.05 compared to cells treated with TGF-β alone.