Figure 4. Ephrin B1 increased inflammatory mediators in rMC-1 cells. rMC-1 cells were grown in normal (5 mM) or high (25 mM) glucose. Some cells in each glucose condition were treated with ephrin B1-Fc. Western blotting was performed for (A) HMGB1 and (B) NLRP3. An ELISA was done for IL-1β. *p<0.05 versus NG, #p<0.05 versus HG. n=5. Data are presented as mean ± SEM.