Figure 5 of Peng, Mol Vis 2024; 30:123-136.


Figure 5. Measurement of Cre activity in the lens of zTg(cryaa:Cre-cryaa:EGFP). A: Schematic map of the pcryaa:RFP-cmlc:EGFP plasmid and images of 2 dpf WT and zTg(cryaa:Cre-cryaa:EGFP; Tg) zebrafish that carried the pcryaa:RFP-cmlc:EGFP plasmids under bright-phase and fluorescence microscopy. RFP fluorescence was specifically expressed in the lens, while GFP was expressed in the heart. B: Schematic map of the pcryaa:loxP-RFP-loxP-cmlc:EGFP plasmid and images of the 2 dpf lenses of WT and zTg(cryaa:Cre-cryaa:EGFP; Tg) zebrafish that were injected with pcryaa:loxP-RFP-loxP-cmlc:EGFP. RFP was specifically expressed in WT zebrafish lenses (upper panel) but not in zTg(cryaa:Cre-cryaa:EGFP) lenses (lower panel).