Figure 6 of Suh, Mol Vis 2024; 30:114-122.


Figure 6. Induction of Hsp72 expression by OPTN-GFP in ARPE-19 cells. RGC-5 cells, HT1080 cells, and human skin fibroblast cells. A: Images for phase-contrast and green fluorescence were separately photographed from ARPE-19 cells transduced with adenoviruses expressing the indicated proteins. Each row represents the same cells. Images presented here are typical of those from three independent experiments. B: Cell lysates made from cells in A were analyzed by immunoblot analysis using the indicated antibodies. The same blot probed for GFP was stripped and re-probed for Hsp72 and tubulin consecutively. C: The quantification of B after normalized with tubulin expression. * denotes p < 0.0001 compared to any other groups. D: Cell lysates made from RGC-5 cells, HT1080 cells, and human skin fibroblast cells were analyzed by immunoblot analysis using the indicated antibodies. The same blot probed for GFP was stripped and re-probed for Hsp72 and tubulin consecutively. E: Quantification of Dafter normalized with tubulin expression. *** denotes p < 0.001 compared to any other groups.