Figure 5. Colocalization and coimmunoprecipitation of OPTN-GFP with Hsp72 in HTM cells. A: Cells transduced with an adenovirus expressing optineurin-GFP were counterstained with DAPI, and Hsp72 (red) was visualized with a Cy-3-conjugated secondary antibody. Two panels represent the same cells. B: Cell lysate made from cells transduced with an adenovirus expressing OPTN-GFP was immunoprecipitated with an anti-GFP antibody and then probed with an anti-Hsp72 antibody and vice versa. 1, input; 2, immunoprecipitation with resin only; 3, immunoprecipitation with resin plus the indicated antibodies.