Figure 3. Confirmation of OPTN-GFP-induced Hsp72 expression by immunocytochemistry in HTM cells. A: Cells transduced with adenoviruses expressing the indicated proteins were counterstained with DAPI, and Hsp72 (red) was visualized with Cy-3-conjugated secondary antibody. Each row represents the same cells. Images presented here are typical of those taken from fifteen fields of at least three independent experiments. Note that the yellowish color of Hsp72 staining is attributed to excessive staining due to its over-expression. B: Quantification of B. * denotes p < 0.05. ** and *** denote p < 0.01 and p < 0.001, respectively.