Figure 2. Induction of Hsp72 expression by GFP-OPTN in HTM cells. A: Images for phase-contrast and green fluorescence were separately photographed from cells transduced with adenoviruses expressing the indicated proteins. Images representative of those from three independent experiments are shown. Each row represents the same cells. B: Cell lysates made from cells in A were analyzed by immunoblot analysis using the indicated antibodies. The same blot probed for GFP was stripped and re-probed for Hsp72 and tubulin consecutively. Two independent experiments were performed with identical results. C: Quantification of B after normalized with tubulin expression.** denotes p < 0.01.