Figure 5. NADPH binding of kangaroo lens µ-crystallin

Summary of results for binding soluble extract of kangaroo lens to blue-sepharose followed by elution with salt and (separately) with 10 mM concentrations of oxidized and reduced nicotinamide adenine dinucleotide cofactors. µ-Crystallin is specifically retained on blue sepharose and is preferentially eluted by NADPH. All lanes are from the same gel. The kangaroo lens extract lane was moved to improve clarity of the figure.