Figure 2. Human µ-crystallin

(a) The 5' end of the human cDNA obtained by 5' RACE. Figure shows the partial cDNA sequence from multiple RACE-derived clones. Genbank Accession number L02950. Conceptual translation is shown in italics.

                               M  S  R  V  P  A  F  L  S  A  A  E  V  E  E  H  L  R  S  S  S   21
agactgaggttagaaggcacaggtggcgagATGAGCCGGGTACCAGCGTTCCTGAGCGCGGCCGAGGTGGAGGAACACCTCCGCAGCTCCAGC  93

 L  L  I  P  P  L  E  T  A  L  A  N  F  S  S  G  P  E  G  G  V  M  Q  P  V  R  T  V  V  P  V   52
CTCCTCATCCCGCCTCTAGAGACGGCCCTGGCCAACTTCTCCAGCGGTCCCGAAGGAGGGGTCATGCAGCCCGTGCGCACCGTGGTGCCGGTG 186

 T  K  H  R  G  Y  L  G  V  M  P  A  Y  S  A  A  E  D  A  L  T  T  K  L  V  T  F  Y  E  D  R   83
ACCAAGCACAGGGGCTACCTGGGGGTCATGCCCGCCTACAGTGCTGCAGAGGATGCACTGACCACCAAGTTGGTCACCTTCTACGAGGACCGC 279

 G  I  T  S  V  V  P  S  H  Q  A  T  V  L  L  F  E  P  S  N  G  T  L  L  A  V  M  D  G  N ... 113
GGCATCACCTCGGTCGTCCCTTCCCACCAGGCTACTGTGCTACTCTTTGAGCCCAGCAATGGCACCCTGCTGGCGGTCATGGATGGAAATG.. 370

(b) Alignment of complete deduced protein sequences for human retina and grey kangaroo (Macropus fuliginosus) lens µ-crystallin. The human sequence is shown and below it are non-identical positions from kangaroo. Dots indicate the location of the peptide used to raise antiserum.

MSRVPAFLSAAEVEEHLRSSSLLIPPLETALANFSSGPEGGVMQPVRTVV  50
  WS    RSED  RY G   I L A  K        S    V      I

PVTKHRGYLGVMPAYSAAEDALTTKLVTFYEDRGITSVVPSHQATVLLFE 100
  A  Q F  I  V   S             GMSP  TA    T   F D

PSNGTLLAVMDGNVITAKRTAAVSAIATKFLKPPSSEVLCILGAGVQAYS 150
    S  SI    I

HYEIFTEQFSFKEVRIWNRTKENAEKFADTVQGEVRVCSSVQEAVAGADV 200
     K               K      Q  K D           T

IITVTLATEPILFGEWVKPGAHINAVGASRPDWRELDDELMKEAVLYVDS 250
     M  K                              I  NC
      .....................
QEAALKESGDVLLSGAEIFAELGEVIKGVKPAHCEKTTVFKSLGMAVEDT 300
R          I             V       R               A

VAAKLIYDSWSSGK 314
     V