Figure 3 of
Shearer, Mol Vis 3:8, 1997.
Figure 3. ß-crystallins proteolytic fragments from selenite nuclear cataract
Cleavage sites on the N-terminal extensions of ß-crystallin polypeptides in the insoluble pellet from selenite nuclear cataract. This figure identifies only those polypeptides in selenite cataract showing the same cleavage sites as found in ß-heavy crystallins incubated with m-calpain in vitro (24). The data were obtained by blotting and N-terminal sequencing the protein spots from two-dimensional electrophoretic gels. (Truncated alpha-A spots are missing from this gel.)