Figure 4. Amino acid sequences of chdlx-3 and chanf mRNA fragments aligned to other dlx- and anf sequences to show homologies.

Sequences identical to mdlx-3 are shown in asterisks (*) and stop codons as vertical hashes (|). The GAVY sequence at the end of other dlx-3 sequences is shown in blue. Chdlx-3 and chanf are denoted by magenta text.

A. The zebrafish (zdlx-3), the newt (nvhbox4), the chicken (chdlx-3 & 5), the mouse sequences (mdlx-1 & 2), and the Drosophila (dll), are shown aligned to the mouse mdlx-3 sequence. The single amino acid insertion of glycine in the Drosophila and newt sequences at the sixth position after the homeodomain is represented as a dot in the other sequences. The chdlx-3 sequence shows 24% homology to the mouse mdlx-1, 27% to mdlx-5, 31% to mdlx-2, 36% to mdlx-3, 33% to zdlx-3 and 29% to nvhbox4 in the sequences shown.

B. The chicken anf (chanf) sequence aligned to the Xenopus (xanf-1 & 2) and mouse (Hesx-1, Rpx) sequences. The chicken sequence is identical to the xanf-1 & 2 in the homeobox sequences and is identical at 55% of the amino acid positions in the 3' coding sequences. It bears 87% identity to the mouse sequences in the homeobox region and 47-52% identity in the 3' coding sequences.

A	
	Homeodomain----(	Carboxyl terminus

	helix-3	      helix-4		6
mdlx3	KIWFQNRR	SKFKKLYK	NGEVP.LEHSPNNSDSMACNSPPSPALWDTSSHSTPAPARNPLPPPLPYSASPNYLDDPTTSWYHTQNLSGPHLQQQPPQPATLHHASPGPPPNPGAVY|
zdlx3	********	********	*****.******A*************V**NNA**SQVNRGQIPQ***SSTPPYMEDYSNHWYQQGSHLQHPV*HPGP*QSVGAVY|
nvhbox4	********	********	*****GM****D**********A**T*****TP*RVQHTQAQPL*HNSSPSYLEDYNPWYHHPQNLSGHLQPPGTMHHTPPGTGAVY|
chdlx3	********	********	*****.**************EGTAF*GVEGLRVRVAKGLLHTCCCAAHT*VNKHL*VFV|
chdlx5	******K*	**I**IM*	***M*.P****SS**P****SPQSP*VW*PQGSSRSIGHHGHGH**AANPSPGS**ES*SAWYPAASP*GSH?QPHGSLOHPLALPSGTIY|
mdlx2	********	*****MW*	S**I*.T*QH*GA*A*PP*A***VS*PASWDFGAPQRM*GGGPGSGGGGAG*SGSSPSSAA*AFLGNYPWYHQASGSASHLQATAPLLHPSQTPQAHHHHHHHHHAGGGAPVSAGTIF|
mdlx1	******K*	******M*	Q*GAA.**G*ALANGRALSAGS*PVPPGWNPNSSSGKGSGSSAGSYV*SYT*WYPSAHQEAMQQPQLM|
dll 	********	**Y**MM*	AAQG*GTNSGMPLGGGGPNPGQHSPNQMHSGGNNGGGSNSGSPSHY**PGH**TPSST*VSELSPQFPPT*LSPPT*A*WDQKP*WIAHK***QM*GYVPQYWYLPETNPSLVTVWPAV|



B
	Homeodomain----(	 Carboxyl-terminus 	

        helix-3       helix-4
xanf-1	QIWFQNRR	AKLKRSHR	ESQFLIVKDSLSSKIQE|
xanf-2	********	********	***************E*|
mRpx	********	**M***R*	*****MA*KPFNPDLLK|
Hesx-1	********	**M***R*	*****MARKPFNPDLLK|
chanf	********	********	*****M**NNST*SLLE|