Figure 4. Bst EII restriction analysis of murine Lim2 Fragment 4 PCR products.

Fragment 4 PCR products from To3/To3, To3/+, and +/+. DNA's were incubated without (-) or with (+) Bst EII restriction endonuclease and then electrophoresed on an agarose gel stained with ethidium bromide. Presence of the sequence is clearly linked to the mutant phenotype and corroborates the sequence data.