Figure 6. A multiple sequence alignment of the human, bovine, and mouse IRBP promoter regions.

Shown here is the alignment from -70 to +1. The numbering shown corresponds to the mouse sequence. Identical bases are shown by the = above each nucleotide. Consensus sequence matches conserved among all three species are: the Ret-1/PCE-I element at -65 to -60, the CRX-binding element (CBE) at -55 to -50, an AP-4 consensus core sequence at -37 to -34, a cETS consensus core at -35 to -31 and another at positions -57 to -54, and an S8 homeodomain is shown by "8888" at -64 to -61. Only the core bases are marked. The criteria for searching the TRANSFAC Database by MatInspector were a match to the core sequence of at least 80% and to the entire consensus sequence of at least 85%. The Genbank entries for human, bovine, and mouse are X53044, M32733, and M32734, respectively.

       ========== = ============ ===   = ===== ===    =  =  =====     == =======
human  GCTTGAATTAGACAGGATTAAAGGC TTACTGGAGCTGGAAGCCTTGCCCC -AACTCAGGAGTTTAGCCCCA
bovine GCTTGAATTAAATAGGATTAAAGGC TTATCAGGGCTGGGAGCTACACCCC -AACTCCTGAGTTTAGCCCCA
mouse  GCTTGAATTAGACAGGATTAAAGGC TTAGCAGAGCTGGAAGCCTCACATC TAACTCCCACATTGAGCCCCA
            PCE-I     -CBE--             AP-4
             8888   cETS                   cETS
       |                         |                         |                   |
     -70                       -45                       -20                  +1