Figure 2 of
Boatright, Mol Vis 3:15, 1997.
Figure 2. Orientation sensitivity of the IRBP promoter.
A -1783 to +101 fragment of the murine IRBP promoter was inserted into the pBLCAT3 reporter vector either in the forward orientation (p1783) or the reverse orientation (p1783rev) and constructs transfected into primary cultures of embryonic day 10 chick retina cells as described in the text. Following four days of incubation, cells were harvested and assayed for CAT activity as a measure of promoter activity. The -1783/+101 insert stimulated CAT activity. Inverting the insert abolished most but not all of this activity. Activity with p1783 is significantly greater than that produced by transfection with the other plasmids (p < 0.05). Activity produced by p1783rev is greater than that produced by pBLCAT3 (p < 0.05). The data are from three experiments. Activities are means of percentage of p1783 ± SEM from individual experiments. Sample size (number of transfected dishes) is indicated in parentheses above error bars.